Cloning and Expression of the G1 Epitope of Bovine Ephemeral Fever Virus G Glycoprotein in Escherichia Coli
نویسنده
چکیده
Bovine ephemeral fever (BEF) is an acute epidemic disease in cattle and water buffalo, spanning tropical and subtropical zones of Asia, Australia, and Africa. In recent years, BEF has been distributed in many provinces of Iran and caused economic losses. In this study the sequence encoding the G1 epitope of bovine ephemeral fever virus (BEFV) G glycoprotein was amplified by PCR, ligated into the prokaryotic expression vector, pMAL-c2X, and subsequently cloned into Rosetta strain of Escherichia coli. After being induced with Isopropyl β-D-1-thiogalactopyranoside, SDS-PAGE analysis showed that a protein of 58 kDa molecular weight, consistent with the expected molecular weight of maltose binding protein-G1 fusion protein, was expressed. Also, dot blot analysis confirmed that the expressed protein has specifically reacted with an anti-BEFV mouse serum. In conclusion, the results indicate that the recombinant G1 epitope expressed in this study could be used as a coating antigen to develop an ELISA for bovine phemeral fever diagnosis.
منابع مشابه
Expression of G1- epitope of bovine ephemeral fever virus in E. coli : A novel candidate to develop ELISA kit
Bovine ephemeral fever is an acute and arthropod-borne viral disease of cattle and water buffalo which occurs seasonally in most of the world tropical and subtropical regions. The epizootic feature of the disease has been reported in Iran with serious economic consequences. The surface glycoprotein G of bovine ephemeral fever virus (BEFV) is composed of 4 antigenic sites (G1-G4) and plays the m...
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Bovine ephemeral fever is an acute and arthropod-borne viral disease of cattle and water buffalo which occurs seasonally in most of the world tropical and subtropical regions. The epizootic feature of the disease has been reported in Iran with serious economic consequences. The surface glycoprotein G of bovine ephemeral fever virus (BEFV) is composed of 4 antigenic sites (G1-G4) and plays the m...
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